Fig 1: Induction of EECs from hiPSCs. a A schematic diagram of the experiment to examine the conditions underlying the differentiation of foregut (FG) into dorsal anterior foregut (dAFG). b An expression analysis of SOX2 and p63 at Day 13 by semi-quantitative RT-PCR. GAPDH was used as an endogenous control. c Immunostaining of SOX2 (green), p63 (red) and Hoechst (blue) at Day 13. Representative fluorescence is shown. Scale bars, 50 μm. d A schematic diagram of the experiment to examine the conditions underlying the differentiation of dAFG into EECs. e Expression analyses of p63, SOX2, CK13, CK5 and PAX9 at Day 21 by semi-quantitative RT-PCR. GAPDH was used as an endogenous control. Total RNA of human normal esophageal tissue was used as a positive control